Journal Article

PreJuSER-16546

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Nanocavity electrode array for recording from electrogenic cells

Hofmann, B.FZJ* ; Kätelhön, E.FZJ* ; Schottdorf, M.FZJ* ; Offenhäusser, A.FZJ* ; Wolfrum, B.FZJ*

2011
RSC Cambridge

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Please use a persistent id in citations: http://hdl.handle.net/2128/7320  doi:10.1039/C0LC00582G

Abstract: We present a new nanocavity device for highly localized on-chip recordings of action potentials from individual cells in a network. Microelectrode recordings have become the method of choice for recording extracellular action potentials from high density cultures or slices. Nevertheless, interfacing individual cells of a network with high resolution still remains challenging due to an insufficient coupling of the signal to small electrodes, exhibiting diameters below 10 µm. We show that this problem can be overcome by a new type of sensor that features an electrode, which is accessed via a small aperture and a nanosized cavity. Thus, the properties of large electrodes are combined with a high local resolution and a good seal resistance at the interface. Fabrication of the device can be performed with state-of-the-art clean room technology and sacrificial layer etching allowing integration of the devices into sensor arrays. We demonstrate the capability of such an array by recording the propagation of action potentials in a network of cardiomyocyte-like cells.

Keyword(s): Action Potentials: physiology (MeSH) ; Animals (MeSH) ; Cell Line, Tumor (MeSH) ; Electrochemical Techniques (MeSH) ; Lab-On-A-Chip Devices (MeSH) ; Mice (MeSH) ; Microelectrodes (MeSH) ; Nanotechnology (MeSH) ; J

Note: The authors thank Marko Banzet for clean room support, Norbert Wolters and Ralph Otto for building the electronic amplifiers and Daniela Esser for preparation of the FIB-cuts. The work was funded by the Young Investigators Program of the Helmholtz Association.

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Contributing Institute(s):

1. Bioelektronik (ICS-8)
2. Bioelektronik (PGI-8)
3. Jülich-Aachen Research Alliance - Fundamentals of Future Information Technology (JARA-FIT)

Research Program(s):

1. Grundlagen für zukünftige Informationstechnologien (P42)
2. BioSoft: Makromolekulare Systeme und biologische Informationsverarbeitung (P45)

Appears in the scientific report 2011

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