Journal Article

PreJuSER-16686

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Global Structure of Forked DNA in Solution Revealed by High-Resolution Single-Molecule FRET

Sabir, T.FZJ* ; Schröder, G. F.FZJ* ; Toulmin, A.FZJ* ; McGlynn, P.FZJ* ; Magennis, S. W.FZJ*

2011
American Chemical Society Washington, DC

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Please use a persistent id in citations: doi:10.1021/ja108626w

Abstract: Branched DNA structures play critical roles in DNA replication, repair, and recombination in addition to being key building blocks for DNA nanotechnology. Here we combine single-molecule multiparameter fluorescence detection and molecular dynamics simulations to give a general approach to global structure determination of branched DNA in solution. We reveal an open, planar structure of a forked DNA molecule with three duplex arms and demonstrate an ion-induced conformational change. This structure will serve as a benchmark for DNA-protein interaction studies.

Keyword(s): Base Sequence (MeSH) ; DNA: chemistry (MeSH) ; Fluorescence Resonance Energy Transfer (MeSH) ; Molecular Dynamics Simulation (MeSH) ; Nucleic Acid Conformation (MeSH) ; Solutions (MeSH) ; Solutions ; DNA ; J

Note: We thank the BBSRC (BB/G00269X/1) for support and Claus Seidel, Ralf Kuhnemuth, Suren Felekyan, Stefan Marawske, Alessandro Valeri, and Evangelos Sisamakis for assistance in developing our MFD setup. We thank Richard Henchman for helpful discussions. S.W.M. acknowledges the award of an EPSRC Advanced Research Fellowship (EP/D073154).

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Contributing Institute(s):

1. Strukturbiochemie (ICS-6)

Research Program(s):

1. Funktion und Dysfunktion des Nervensystems (P33)
2. BioSoft: Makromolekulare Systeme und biologische Informationsverarbeitung (P45)

Appears in the scientific report 2011

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