Journal Article

FZJ-2014-07230

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Simultaneous monitoring of cerebral metal accumulation in an experimental model of Wilson’s disease by laser ablation inductively coupled plasma mass spectrometry

Boaru, S. (Corresponding Author) ; Merle, U. ; Uerlings, R. ; Zimmermann, A.FZJ* ; Weiskirchen, S. ; Matusch, A.FZJ* ; Stremmel, W. ; Weiskirchen, R.

2014
BioMed Central London

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Please use a persistent id in citations: http://hdl.handle.net/2128/9099  doi:10.1186/1471-2202-15-98

Abstract: Neuropsychiatric affection involving extrapyramidal symptoms is a frequent component of Wilson’s disease (WD). WD is caused by a genetic defect of the copper (Cu) efflux pump ATPase7B. Mouse strains with natural or engineered transgenic defects of the Atp7b gene have served as model of WD. These show a gradual accumulation and concentration of Cu in liver, kidneys, and brain. However, still little is known about the regional distribution of Cu inside the brain, its influence on other metals and subsequent pathophysiological mechanisms. We have applied laser ablation inductively coupled plasma mass spectrometry and performed comparative metal bio-imaging in brain sections of wild type and Atp7b null mice in the age range of 11–24 months. Messenger RNA and protein expression of a panel of inflammatory markers were assessed using RT-PCR and Western blots of brain homogenates.

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Contributing Institute(s):

1. Analytik (ZEA-3)

Research Program(s):

1. 331 - Signalling Pathways and Mechanisms in the Nervous System (POF2-331) (POF2-331)
2. 89572 - (Dys-)function and Plasticity (POF2-89572) (POF2-89572)

Appears in the scientific report 2014

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Database coverage:
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