Journal Article

PreJuSER-19217

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Combination of PAGE and LA-ICP-MS as an analytical workflow in metallomics: state of the art, new quantification strategies, advantages ans limitations

Sussulini, A.FZJ* ; Becker, J. S.FZJ*

2011
RSC Publ. Cambridge

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Please use a persistent id in citations: http://hdl.handle.net/2128/7386  doi:10.1039/c1mt00116g

Abstract: Metallomics (more specifically, metalloproteomics) is an emerging field that encompasses the role, uptake, transport and storage of trace metals, which are essential to preserve the functions of proteins within a biological system. The current strategies for metal-binding and metalloprotein analysis based on the combination of polyacrylamide gel electrophoresis (PAGE) and laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) are discussed in this review. The advantages, limitations and the most recently developed and applied quantification approaches for this methodology are also described.

Keyword(s): Animals (MeSH) ; Electrophoresis, Polyacrylamide Gel: methods (MeSH) ; Humans (MeSH) ; Mass Spectrometry: methods (MeSH) ; Metalloproteins: chemistry (MeSH) ; Metalloproteins: isolation & purification (MeSH) ; Metals: analysis (MeSH) ; Metalloproteins ; Metals

Note: Record converted from VDB: 12.11.2012

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Contributing Institute(s):

1. Zentralabteilung für Chemische Analysen (ZCH)

Research Program(s):

1. Funktion und Dysfunktion des Nervensystems (P33)

Appears in the scientific report 2011

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Database coverage:

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