Journal Article

PreJuSER-19781

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png AtSUC2 has a role for sucrose retrieval along the phloem pathway: Evidence from carbon-11 tracer studies

Gould, N. ; Thorpe, M. R. ; Pritchard, J. ; Christeller, J. I. ; Williams, L. E. ; Roeb, G.FZJ* ; Schurr, U.FZJ* ; Minchin, P. E. H.FZJ*

2012
Elsevier Science Amsterdam [u.a.]

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Please use a persistent id in citations: doi:10.1016/j.plantsci.2011.12.018

Abstract: The location of the phloem within a plant, and its vulnerability to disruption, make it a difficult tissue to study and therefore non-invasive studies of phloem functionality are important. Here we compare, phloem transport, measured non-invasively, in wild type Arabidopsis thaliana, and transposon-insertion mutants for AtSUC1 or AtSUC2, giving in vivo information on the importance of these sucrose transporters for phloem transport. The suc2 mutant showed an increase in both phloem leakage and transport time, consistent with reduced sucrose uptake into both transport and collection phloem. The results are consistent with the AtSUC2 transporter being important for retrieval of leaked sucrose in the transport phloem of Arabidopsis. There was no difference in phloem transport properties between the wild type and the suc1 mutants, implying that the AtSUC1 transporter does not play a significant role within the transport phloem of Arabidopsis under the conditions of our study.

Keyword(s): Arabidopsis: genetics (MeSH) ; Arabidopsis: metabolism (MeSH) ; Biological Transport (MeSH) ; Carbon: metabolism (MeSH) ; Carbon Radioisotopes: diagnostic use (MeSH) ; Gene Expression Regulation, Plant: genetics (MeSH) ; Gene Knockout Techniques (MeSH) ; Membrane Transport Proteins: genetics (MeSH) ; Membrane Transport Proteins: metabolism (MeSH) ; Mutagenesis, Insertional (MeSH) ; Phloem: metabolism (MeSH) ; Plant Proteins: genetics (MeSH) ; Plant Proteins: metabolism (MeSH) ; Sucrose: metabolism (MeSH) ; Carbon Radioisotopes ; Membrane Transport Proteins ; Plant Proteins ; sucrose transport protein, plant ; Sucrose ; Carbon ; J ; C-11 tracer (auto) ; Phloem leakage (auto) ; Phloem transit times (auto) ; Sucrose transporter (auto) ; Transposon mutagenesis (auto)

Note: This work began in New Zealand using the <SUP>11</SUP>C facility for plant science maintained by the Geological and Nuclear Sciences Crown Research Institute in Lower Hutt, and finished in Germany at the Phytosphere Institute (now Plant Sciences) in the Forschungszentrum Julich. Mr. Chris Purcell in New Zealand, and Mr. Marko Dautzeuberg in Germany, provided exemplary support at all hours. Dr. Laurence Gatehouse and Helen Boldingh (Plant & Food Research) provided valuable comments on the manuscript. Financial support was provided by the New Zealand Marsden Fund (Contract Number UOW003) and by the Forschungszentrum Julich. Peter Minchin and Michael Thorpe wish to thank colleagues at both locations for their support and encouragement during a difficult period when their employment was terminated and the <SUP>11</SUP>C-facility in New Zealand was closed.

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Contributing Institute(s):

1. Pflanzenwissenschaften (IBG-2)

Research Program(s):

1. Terrestrische Umwelt (P24)

Appears in the scientific report 2012

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Database coverage:
; BIOSIS Previews ; Current Contents - Agriculture, Biology and Environmental Sciences ; Current Contents - Life Sciences ; JCR ; NCBI Molecular Biology Database ; Nationallizenz ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection

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