Journal Article

PreJuSER-19969

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png iHADAMAC: a complementary tool for sequential resonance assignment of globular and highly disordered protein

Feuerstein, S.FZJ* ; Plevin, M. J. ; Willbold, D.FZJ* ; Brutscher, B.

2012
Elsevier Amsterdam [u.a.]

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Please use a persistent id in citations: doi:10.1016/j.jmr.2011.10.019

Abstract: An experiment, iHADAMAC, is presented that yields information on the amino-acid type of individual residues in a protein by editing the (1)H-(15)N correlations into seven different 2D spectra, each corresponding to a different class of amino-acid types. Amino-acid type discrimination is realized via a Hadamard encoding scheme based on four different spin manipulations as recently introduced in the context of the sequential HADAMAC experiment. Both sequential and intra-residue HADAMAC experiments yield highly complementary information that greatly facilitate resonance assignment of proteins with high frequency degeneracy, as demonstrated here for a 188-residue intrinsically disordered protein fragment of the hepatitis C virus protein NS5A.

Keyword(s): Algorithms (MeSH) ; Magnetic Resonance Spectroscopy: methods (MeSH) ; Proteins: chemistry (MeSH) ; Proteins: ultrastructure (MeSH) ; Software (MeSH) ; Proteins ; J ; NMR (auto) ; Protein (auto) ; IDP (auto) ; Sequential resonance assignment (auto) ; HADAMAC (auto)

Note: We thank I. Ayala (IBS Grenoble) for help with the production of the protein samples used for this study, and S. Hoffmann (FZ Julich) for stimulating discussion on the NS5A project. This work has been supported in part by grants from the European Commission (FP7-I3-BIO-NMR contract No. 261862, FP7-ITN-IDPbyNMR contract No. 264257, and FPR-IRG-2008 contract No. 231082), and financial support from ARC to M.J.P. and from CEA to S.F.

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Contributing Institute(s):

1. Strukturbiochemie (ICS-6)

Research Program(s):

1. Funktion und Dysfunktion des Nervensystems (P33)
2. BioSoft: Makromolekulare Systeme und biologische Informationsverarbeitung (P45)
3. IDPBYNMR - High resolution tools to understand the functional role of protein intrinsic disorder (264257) (264257)
4. ONCOMIRNA-BIOGENESIS - Biogenesis of Oncogenic MicroRNAs : from the structure of the microRNA processing complexes to the inhibition of the maturation of human oncogenes (231082) (231082)

Appears in the scientific report 2012

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Database coverage:
; Current Contents - Life Sciences ; Current Contents - Social and Behavioral Sciences ; JCR ; NCBI Molecular Biology Database ; Nationallizenz ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection

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