Journal Article

FZJ-2015-03499

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Permeation through the Cell Membrane of a Boron-Based β-Lactamase Inhibitor

Minozzi, M. ; Lattanzi, G. (Corresponding Author) ; Benz, R. ; Costi, M. P. ; Venturelli, A. ; Carloni, P.FZJ*

2011
PLoS Lawrence, Kan.

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Please use a persistent id in citations: http://hdl.handle.net/2128/8785  doi:10.1371/journal.pone.0023187

Abstract: Bacteria express beta-lactamases to counteract the beneficial action of antibiotics. Benzo[b]-thiophene-2-boronic acid (BZB) derivatives are β-lactamase inhibitors and, as such, promising compounds to be associated with β-lactam antibacterial therapies. The uncharged form of BZB, in particular, is suggested to diffuse through the outer membrane of Gram negative bacteria. In this study, through the combination of electrophysiological experiments across reconstituted PC/n-decane bilayers and metadynamics-based free energy calculations, we investigate the permeation mechanism of boronic compounds. Our experimental data establish that BZB passes through the membrane, while computer simulations provide hints for the existence of an aqueous, water-filled monomolecular channel. These findings provide new perspectives for the design of boronic acid derivatives with high membrane permeability.

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Contributing Institute(s):

1. GRS (GRS)
2. Computational Biomedicine (IAS-5)

Research Program(s):

1. 899 - ohne Topic (POF2-899) (POF2-899)

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Database coverage:
; ; ; ; BIOSIS Previews ; IF < 5 ; JCR ; NCBI Molecular Biology Database ; SCOPUS ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection ; Zoological Record

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