Journal Article

FZJ-2015-04422

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Ground state structure of D75N mutant of sensory rhodopsin II in complex with its cognate transducer

Ishchenko, A. ; Round, E. ; Borshchevskiy, V. ; Grudinin, S. ; Gushchin, I.FZJ* ; Klare, J. P. ; Balandin, T.FZJ* ; Remeeva, A. ; Engelhard, M. ; Büldt, G.FZJ* ; Gordeliy, V. (Corresponding Author)FZJ*

2013
Elsevier New York, NY [u.a.]

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Please use a persistent id in citations: doi:10.1016/j.jphotobiol.2013.03.008

Abstract: The complex of sensory rhodopsin II (NpSRII) with its cognate transducer (NpHtrII) mediates negative phototaxis in halobacteria Natronomonas pharaonis. Upon light activation NpSRII triggers, by means of NpHtrII, a signal transduction chain homologous to the two component system in eubacterial chemotaxis. Here we report on the crystal structure of the ground state of the mutant NpSRII-D75N/NpHtrII complex in the space group I212121. Mutations of this aspartic acid in light-driven proton pumps dramatically modify or/and inhibit protein functions. However, in vivo studies show that the similar D75N mutation retains functionality of the NpSRII/NpHtrII complex. The structure provides the molecular basis for the explanation of the unexpected observation that the wild and the mutant complexes display identical physiological response on light excitation.

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Contributing Institute(s):

1. Molekulare Biophysik (ICS-5)
2. Strukturbiochemie (ICS-6)

Research Program(s):

1. 452 - Structural Biology (POF2-452) (POF2-452)

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Database coverage:
; BIOSIS Previews ; Current Contents - Life Sciences ; IF < 5 ; JCR ; NCBI Molecular Biology Database ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection ; Zoological Record

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