Journal Article

FZJ-2015-05001

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Functionalisation and immobilisation of an Au(110) surface via uracil and 2-thiouracil anchored layer

Plekan, O. ; Feyer, V. (Corresponding author)FZJ* ; Cassidy, A. ; Lyamayev, V. ; Tsud, N. ; Ptasińska, S. ; Reiff, S. ; Acres, R. G. ; Prince, K. C.

2015
RSC Publ. Cambridge

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Please use a persistent id in citations: http://hdl.handle.net/2128/8989  doi:10.1039/C5CP01886B

Abstract: We study surface functionalisation by uracil and 2-thiouracil, and immobilisation of several DNA moieties on functionalised gold surfaces. The combination of X-ray photoelectron and near-edge X-ray absorption spectroscopy allowed us to obtain a complete understanding of complex interfacial processes, starting from adsorption of biomolecules onto the metallic surface and progressing towards a specific surface functionality for interactions with other biologically related adsorbates. Au(110) surfaces were functionalised by deposition of uracil and 2-thiouracil molecules under vacuum conditions, and then tested for their selectivity by immobilisation of different DNA moieties deposited from aqueous solutions. We observed that adenine, adenosine, and RNA polymer (polyadenylic acid) from saturated solutions were immobilized successfully on the 2-thiouracil, but those from dilute (1%) solutions were not. However, cytosine failed to adsorb even from saturated solution. The chemical states of the biologically related adsorbates were investigated and the geometrical orientation of uracil and 2-thiouracil on the Au(110) surface was determined using both spectroscopic techniques.

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Contributing Institute(s):

1. Elektronische Eigenschaften (PGI-6)
2. JARA-FIT (JARA-FIT)

Research Program(s):

1. 522 - Controlling Spin-Based Phenomena (POF3-522) (POF3-522)

Appears in the scientific report 2015

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Database coverage:
; ; Current Contents - Physical, Chemical and Earth Sciences ; IF < 5 ; JCR ; NCBI Molecular Biology Database ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection

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