Journal Article

FZJ-2015-05215

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Characterization of the mechanical properties of HL-1 cardiomyocytes with high throughput magnetic tweezers

Chen, L.FZJ* ; Maybeck, V.FZJ* ; Offenhäusser, A.FZJ* ; Krause, H.-J. (Corresponding author)

2015
American Inst. of Physics Melville, NY

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Please use a persistent id in citations: http://hdl.handle.net/2128/17242  doi:10.1063/1.4928322

Abstract: We characterized the mechanical properties of cardiomyocyte-like HL-1 cells using our recently developed multi-pole magnetic tweezers. With the optimized design, both high force and high throughput are achieved at the same time. Force up to 100 pN can be applied on a 1 μm diameter superparamagnetic bead in a workspace with 60 μm radius, which is encircled symmetrically by 3 sharp magnetic tips. By adjusting the coil currents, both the strength and direction of force can be controlled. The result shows that both viscosity and shear elastic modulus of HL-1 cells exhibit an approximately log-normal distribution. The cells became stiffer as they matured, consistent with a transition from proliferating cells to contractile muscle tissue. Moreover, the mechanical properties of HL-1 cells show high heterogeneity, which agrees well with their physiological structure.

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Contributing Institute(s):

1. Bioelektronik (PGI-8)
2. Bioelektronik (ICS-8)
3. JARA-FIT (JARA-FIT)

Research Program(s):

1. 552 - Engineering Cell Function (POF3-552) (POF3-552)
2. 553 - Physical Basis of Diseases (POF3-553) (POF3-553)

Appears in the scientific report 2015

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Database coverage:
; ; Current Contents - Physical, Chemical and Earth Sciences ; IF < 5 ; JCR ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection

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