Journal Article PreJuSER-21070

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Structure of an intermediate state in protein folding and aggregation

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2012
American Association for the Advancement of Scienc Washington, DC [u.a.]

Science 336, 362 - 366 () [10.1126/science.1214203]

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Abstract: Protein-folding intermediates have been implicated in amyloid fibril formation involved in neurodegenerative disorders. However, the structural mechanisms by which intermediates initiate fibrillar aggregation have remained largely elusive. To gain insight, we used relaxation dispersion nuclear magnetic resonance spectroscopy to determine the structure of a low-populated, on-pathway folding intermediate of the A39V/N53P/V55L (A, Ala; V, Val; N, Asn; P, Pro; L, Leu) Fyn SH3 domain. The carboxyl terminus remains disordered in this intermediate, thereby exposing the aggregation-prone amino-terminal β strand. Accordingly, mutants lacking the carboxyl terminus and thus mimicking the intermediate fail to safeguard the folding route and spontaneously form fibrillar aggregates. The structure provides a detailed characterization of the non-native interactions stabilizing an aggregation-prone intermediate under native conditions and insight into how such an intermediate can derail folding and initiate fibrillation.

Keyword(s): Amyloid: chemistry (MeSH) ; Animals (MeSH) ; Chickens (MeSH) ; Hydrogen Bonding (MeSH) ; Models, Molecular (MeSH) ; Molecular Dynamics Simulation (MeSH) ; Mutant Proteins: chemistry (MeSH) ; Nuclear Magnetic Resonance, Biomolecular (MeSH) ; Protein Conformation (MeSH) ; Protein Folding (MeSH) ; Protein Structure, Secondary (MeSH) ; Proto-Oncogene Proteins c-fyn: chemistry (MeSH) ; Proto-Oncogene Proteins c-fyn: genetics (MeSH) ; Thermodynamics (MeSH) ; src Homology Domains (MeSH) ; Amyloid ; Mutant Proteins ; Proto-Oncogene Proteins c-fyn ; J


Note: We thank J. Forman-Kay and A. Davidson for providing laboratory space. S.S. and L.E.K. hold Canada Research Chairs in Biochemistry. P.R. was funded by a Gates Cambridge Scholarship and an NSF Graduate Research Fellowship, and P.W. is funded by a Natural Sciences and Engineering Research Council of Canada (NSERC) Canada Graduate Scholarship. This work was supported by grants from the NSERC and the Canadian Institutes of Health Research (L.E.K). The ensembles of 10 structures each representing the A39V/N53P/V55L Fyn SH3 domain native state from Ser<SUP>1</SUP> to Asp<SUP>59</SUP> and the folding intermediate from Ser<SUP>1</SUP> to Ala<SUP>56</SUP> have been deposited with the Protein Data Bank (accession codes 2LP5 and 2L2P, respectively).

Contributing Institute(s):
  1. Strukturbiochemie (ICS-6)
Research Program(s):
  1. Funktion und Dysfunktion des Nervensystems (P33)
  2. BioSoft: Makromolekulare Systeme und biologische Informationsverarbeitung (P45)

Appears in the scientific report 2012
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 Record created 2012-11-13, last modified 2020-04-02



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