Journal Article

FZJ-2015-06448

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Size of submicrometer particles measured by FCS: Correction of the confocal volume

Deptuła, T. ; Buitenhuis, J.FZJ* ; Jarzębski, M.Extern* ; Patkowski, A. ; Gapinski, J. (Corresponding author)

2015
ACS Publ. Washington, DC

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Please use a persistent id in citations: doi:10.1021/acs.langmuir.5b01225

Abstract: When fluorescence correlation spectroscopy (FCS) in combination with a confocal microscope is used to determine the hydrodynamic radius a of particles comparable to or larger than the linear size σ of the confocal volume of the microscope, a correction must be used that depends on the a2/σ2 ratio and the distribution of the dye within the particle. Here we present the experimental validation of the theoretically predicted approximate correction necessary for appropriate measurements of the size of uniformly fluorescently labeled spheres of radius comparable to the size of the confocal volume. We also test the approximate correction formula for different ranges of the a/σ ratio and propose a simple procedure to obtain the correct nanoparticle size from such a measurement.

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Contributing Institute(s):

1. Weiche Materie (ICS-3)

Research Program(s):

1. 551 - Functional Macromolecules and Complexes (POF3-551) (POF3-551)

Appears in the scientific report 2015

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Database coverage:
; Current Contents - Physical, Chemical and Earth Sciences ; IF < 5 ; JCR ; NCBI Molecular Biology Database ; Nationallizenz ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection

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