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| Hauptseite > Publikationsdatenbank > A high-resolution reference map for cytoplasmic and membrane-associated proteins of Corynebacterium glutamicum |
| Hauptseite > Publikationsdatenbank > A high-resolution reference map for cytoplasmic and membrane-associated proteins of Corynebacterium glutamicum |
| Journal Article | PreJuSER-27326 |
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2001
Wiley-Blackwell
Weinheim
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Please use a persistent id in citations: doi:10.1002/1522-2683(200112)22:20<4404::AID-ELPS4404>3.0.CO;2-2
Abstract: We present a high-resolution reference map for soluble proteins obtained from Corynebacterium glutamicum cells grown in glucose minimal medium. The analysis window covers the p/ range from 4-6 and the molecular mass range from 5-100 kDa. Using overlapping narrow immobilized pH gradients for isoelectric focusing, 970 protein spots were detected after second-dimensional separation on SDS-polyacrylamide gels and colloidal Coomassie-staining. By tryptic peptide mass fingerprinting 169 protein spots were identified, representing 152 different proteins including many enzymes involved in central metabolism (18), amino acid biosynthesis (24) and nucleotide biosynthesis (11). Thirty-five of the identified proteins have no known function. A comparison of the observed and the expected physicochemical properties of the identified proteins indicated that nine proteins were covalently modified, since variants with apparently identical molecular mass, but differing p/ were detected. The N-termini of eight proteins were determined by post-source decay (PSD) analysis of selected peptides. In addition to the soluble proteins, a map of the membrane-bound proteins within the p/ range 4-7 is presented, which contains 660 protein spots, 22 of which were identified, representing 13 different proteins.
Keyword(s): J ; Corynebacterium glutamicum (auto) ; proteome (auto) ; two-dimensional gel electrophoresis (auto) ; peptide mass fingerprint (auto) ; mass spectrometry (auto)
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