Journal Article

PreJuSER-31243

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Use of chlorophyll fluorescence in metal-stress research: a case study with the green microalga Scenedesmus

Mallick, N. ; Mohn, F. H.FZJ*

2003
Elsevier Amsterdam

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Please use a persistent id in citations: doi:10.1016/S0147-6513(02)00122-7

Abstract: Metal toxicity on the photosystem 11 (PS 11) photochemistry of the green microalga Scenedesmus obliquus was investigated in vivo using a pulse-amplitude-modulated fluorometer. The results demonstrated that the test metals (copper, chromium, nickel, cadmium, and zinc) inhibited PS II photochemistry substantially, which was clearly evident for F-0, F-v/F-m, qN, and qP following 12 h of metal exposure, whereas F-m, F-v/2, and F-0/F-v depicted significant alteration after only I h of treatment. Thus, metals are not only found to affect the initial and maximal fluorescence, maximum quantum yield, photochemical and nonphotochemical quenching, and plastoquionone pool but the water-splitting apparatus was also significantly altered under metal stress. Among all these fluorescence characteristics, the F-0/F-v registered the highest sensitivity to all the five test metals, thus indicating that the water-splitting apparatus of the oxidizing side of PS II is the primary site of action of toxic metals. This study therefore, concludes that F-0/F-v ratio can be used as a powerful tool in metal-stress research. (C) 2003 Elsevier Science (USA). All rights reserved.

Keyword(s): J ; chlorophyll fluorescence (auto) ; metal stress (auto) ; Scenedesmus (auto)

Note: Record converted from VDB: 12.11.2012

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Contributing Institute(s):

1. Phytosphäre (ICG-III)

Research Program(s):

1. Chemie und Dynamik der Geo-Biosphäre (U01)

Appears in the scientific report 2003

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