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| Hauptseite > Publikationsdatenbank > Practical applications of hydrogenase I from Pyrococcus furiosus for NADPH generation and regeneration |
| Hauptseite > Publikationsdatenbank > Practical applications of hydrogenase I from Pyrococcus furiosus for NADPH generation and regeneration |
| Journal Article | PreJuSER-31350 |
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2003
Elsevier Science
Amsterdam [u.a.]
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Please use a persistent id in citations: doi:10.1016/S1381-1177(03)00071-7
Abstract: The soluble hydrogenase I (H-2:NADP(+) oxidoreductase, EC 1.18.99.1) from the marine hyperthermophilic strain of the archaeon Pyrococcus furiosus was partially purified by anion-exchange chromatography. This P furiosus hydrogenase I preparation (PF H(2)ase I) has been used as biocatalyst in the enzymatic production and regeneration of beta-1,4-nicotinamide adenindinucleotide phosphate, reduced form (NADPH), utilizing cheap molecular hydrogen and forming protons as the only side-product. Any excess of dihydrogen can be removed easily. It could be demonstrated, that this hyperthermophilic hydrogenase exhibits a high stability under reaction conditions. Generation as well as regeneration of NADPH were performed in batch and repetitive batch experiments with recyclisation of the biocatalyst. In two repetitive batch-series 6.2 g l(-1) NADPH could be produced with a total turnover number (ttn: mol produced NADPH/mol consumed enzyme) of 10,000. Utilizing the thermophilic NADPH-dependent alcohol dehydrogenase from Thermoanaerobium spec. (ADH M) coupled to the PF H(2)ase I in situ NADPH-regenerating system, two prochiral model substrates, acetophenone and (2S)-hydroxy-1-phenyl-propanone (HPP), were quantitatively reduced to the corresponding (S)-alcohol and (1R,2S)-diol. An e.e. >99.5% and d.e. >98%, respectively, with total turnover numbers (ttn: mol product/mol consumed cofactor NADP(+)) of 100 and 160 could be reached. (C) 2003 Elsevier B.V. All rights reserved.
Keyword(s): J ; Pyrococcus furiosus hydrogenase (auto) ; NADPH (auto) ; cofactor regeneration (auto) ; dihydrogen (auto) ; enantioselective reduction (auto)
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