Journal Article

PreJuSER-43304

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Oriented attachment and membrane reconstitution of His-tagged cytochrome c oxidase to a gold electrode: in-situ monitoring by Surface Enhanced Infrared Absorption Spectroscopy

Ataka, K.FZJ* ; Giess, F. ; Knoll, W. ; Naumann, R. ; Haber-Pohlmeier, S.FZJ* ; Richter, B. ; Heberle, J.FZJ*

2004
American Chemical Society Washington, DC

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Please use a persistent id in citations: http://hdl.handle.net/2128/703  doi:10.1021/ja045951h

Abstract: A novel concept is introduced for the oriented incorporation of membrane proteins into solid supported lipid bilayers. Recombinant cytochrome c oxidase solubilized in detergent was immobilized on a chemically modified gold surface via the affinity of its histidine-tag to a nickel-chelating nitrilo-triacetic acid (NTA) surface. The oriented protein monolayer was reconstituted into the lipid environment by detergent substitution. The individual steps of the surface modification, including (1) chemical modification of the gold support, (2) adsorption of the protein, and (3) reconstitution of the lipid bilayer, were followed in situ by means of surface-enhanced infrared absorption spectroscopy (SEIRAS) and accompanied by normal-mode analysis. The high surface sensitivity of SEIRAS allows for the identification of each chemical reaction process within the monolayer at the molecular level. Finally, full functionality of the surface-tethered cytochrome c oxidase was demonstrated by cyclic voltammetry after binding of the natural electron donor cytochrome c.

Keyword(s): Electrochemistry (MeSH) ; Electron Transport Complex IV: chemistry (MeSH) ; Enzymes, Immobilized: chemistry (MeSH) ; Gold: chemistry (MeSH) ; Histidine: chemistry (MeSH) ; Lipid Bilayers: chemistry (MeSH) ; Models, Molecular (MeSH) ; Nitrilotriacetic Acid: chemistry (MeSH) ; Recombinant Proteins: chemistry (MeSH) ; Spectroscopy, Fourier Transform Infrared: methods (MeSH) ; Surface Properties (MeSH) ; Enzymes, Immobilized ; Lipid Bilayers ; Recombinant Proteins ; Nitrilotriacetic Acid ; Histidine ; Gold ; Electron Transport Complex IV ; J

Note: Record converted from VDB: 12.11.2012

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Contributing Institute(s):

1. Biologische Strukturforschung (IBI-2)

Research Program(s):

1. Neurowissenschaften (L01)

Appears in the scientific report 2004

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Database coverage:

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