Journal Article

PreJuSER-53598

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Surface sticking and lateral diffusion of lipids in supported bilayers

Dertinger, T.FZJ* ; von der Hocht, I.FZJ* ; Benda, A. ; Hof, M. ; Enderlein, J.FZJ*

2006
ACS Publ. Washington, DC

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Please use a persistent id in citations: http://hdl.handle.net/2128/687  doi:10.1021/la061389s

Abstract: The diffusion of fluorescently labeled lipids in supported bilayers is studied using two different methods: Z-scan fluorescence correlation spectroscopy (z-scan FCS) and two-focus fluorescence correlation spectroscopy (2f-FCS). It is found that the data can be fitted consistently only when taking into account partial sticking of the labeled lipids to the supporting glass surface. A kinetic reaction-diffusion model is developed and applied to the data. We find a very slow sticking rate which, however, when neglected, leads to strongly varying estimates of the free diffusion coefficient. The study reveals a strong sensitivity of FCS on even slight binding/unbinding kinetics of the labeled molecules, which has significance for related diffusion measurements in cellular lipid membranes.

Keyword(s): Diffusion (MeSH) ; Lipid Bilayers: chemistry (MeSH) ; Surface Properties (MeSH) ; Lipid Bilayers ; J

Note: Record converted from VDB: 12.11.2012

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Contributing Institute(s):

1. Zelluläre Signalverarbeitung (IBI-1)

Research Program(s):

1. Funktion und Dysfunktion des Nervensystems (P33)

Appears in the scientific report 2006

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Database coverage:

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