guest ::
| Home > Publications database > Stimulating natural defenses in poplar clones (OP-367) increases plant metabolism of carbon tetrachloride |
| Home > Publications database > Stimulating natural defenses in poplar clones (OP-367) increases plant metabolism of carbon tetrachloride |
| Journal Article | PreJuSER-53637 |
; ;
2006
Taylor & Francis
Philadelphia, Pa.
This record in other databases: 
Please use a persistent id in citations: doi:10.1080/15226510600846780
Abstract: Groundwater contamination by carbon tetrachloride (CCl4) presents a health risk as a potential carcinogen and pollutant that is capable of depleting the ozone layer. Although use of poplar trees in a phytoremediation capacity has proven to be cost effective for cleaning contaminated sites, minimizing leaf emission of volatile contaminants remains a pressing issue. We hypothesized that recently fixed carbon plays a key role in CCl4 metabolism in planta yielding nonvolatile trichloroacetic acid (TCA) and that the extent of this metabolism can be altered by heightening plant defenses. Labeling intact leaves with (11)CO2 (t 1/2 20.4 m) can test this hypothesis, because the extremely short half-life of the tracer reflects only those processes involving recently fixed carbon. Using radio-HPLC analysis, we observed [(11)C]TCA from leaf extract from poplar clones (OP-367) whose roots were exposed to a saturated solution of CCl4 (520 ppm). Autoradiography of [(11)C]photosynthate showed increased leaf export and partitioning to the apex within 24 h of CCl4 exposure, suggesting that changes in plant metabolism and partitioning of recently fixed carbon occur rapidly. Additionally, leaf CCl4 emissions were highest in the morning, when carbon pools are low, suggesting a link between contaminant metabolism and leaf carbon utilization. Further, treatment with methyljasmonate, a plant hormone implicated in defense signal transduction, reduced leaf CCl4 emissions two-fold due to the increased formation of TCA.
Keyword(s): Acetic Acids: pharmacology (MeSH) ; Carbon Radioisotopes: analysis (MeSH) ; Carbon Tetrachloride: metabolism (MeSH) ; Chromatography, High Pressure Liquid (MeSH) ; Cyclopentanes: pharmacology (MeSH) ; Oxylipins (MeSH) ; Photosynthesis: drug effects (MeSH) ; Plant Growth Regulators: pharmacology (MeSH) ; Plant Leaves: metabolism (MeSH) ; Populus: drug effects (MeSH) ; Populus: metabolism (MeSH) ; Trichloroacetic Acid: metabolism (MeSH) ; Acetic Acids ; Carbon Radioisotopes ; Cyclopentanes ; Oxylipins ; Plant Growth Regulators ; methyl jasmonate ; Carbon Tetrachloride ; Trichloroacetic Acid ; J ; methyl jasmonate (auto) ; carbon-11 (auto) ; phytoremediation (auto) ; plant defenses (auto) ; carbon tetrachloride (auto) ; metabolism (auto) ; trichloroacetic acid (auto)
|
The record appears in these collections: |