Transient binding of CO to Cu(B) in cytochrome c oxidase is dynamically linked to structural changes around a carboxyl group : a time-resolved step-scan Fourier transform infrared investigation

Heitbrink, D.; Sigurdson, H.; Bolwien, C.; Heberle, J.; Brzezinski, P.

Journal Article

Transient binding of CO to Cu(B) in cytochrome c oxidase is dynamically linked to structural changes around a carboxyl group : a time-resolved step-scan Fourier transform infrared investigation

Heitbrink, D.FZJ* ; Sigurdson, H. ; Bolwien, C.FZJ* ; Brzezinski, P. ; Heberle, J.FZJ*

2002
Rockefeller Univ. Press New York, NY

Biophysical journal 82, 1 - 10 (2002)

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Please use a persistent id in citations: http://hdl.handle.net/2128/718

Abstract: The redox-driven proton pump cytochrome c oxidase is that enzymatic machinery of the respiratory chain that transfers electrons from cytochrome c to molecular oxygen and thereby splits molecular oxygen to form water. To investigate the reaction mechanism of cytochrome c oxidase on the single vibrational level, we used time-resolved step-scan Fourier transform infrared spectroscopy and studied the dynamics of the reduced enzyme after photodissociation of bound carbon monoxide across the midinfrared range (2300-950 cm(-1)). Difference spectra of the bovine complex were obtained at -20degreesC with 5 mus time resolution. The data demonstrate a dynamic link between the transient binding of CO to Cu-B and changes in hydrogen bonding at the functionally important residue E(I-286). Variation of the pH revealed that the pK(a) of E(I-286) is >9.3 in the fully reduced CO-bound oxidase. Difference spectra of cytochrome c oxidase from beef heart are compared with those of the oxidase isolated from Rhodobacter sphaeroides. The bacterial enzyme does not show the environmental change in the vicinity of E(I-286) upon CO dissociation. The characteristic band shape appears, however, in redox-induced difference spectra of the bacterial enzyme but is absent in redox-induced difference spectra of mammalian enzyme. In conclusion, it is demonstrated that the dynamics of a large protein complex such as cytochrome c oxidase can be resolved on the single vibrational level with microsecond Fourier transform infrared spectroscopy. The applied methodology provides the basis for future investigations of the physiological reaction steps of this important enzyme.

Keyword(s): J

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