Journal Article

PreJuSER-62893

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png An indole binding site is a major determinant of the ligand specificity of the GABA type A receptor-associated protein GABARAP

Thielmann, Y.FZJ* ; Mohrlüder, J.FZJ* ; Koenig, B. W.FZJ* ; Stangler, T. ; Hartmann, R.FZJ* ; Becker, K.FZJ* ; Höltje, H.-D. ; Willbold, D.FZJ*

2008
Wiley-VCH Weinheim

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Please use a persistent id in citations: doi:10.1002/cbic.200800117

Abstract: The role of tryptophan as a key residue for ligand binding to the ubiquitin-like modifier GABA(A) receptor associated protein (GABARAP) was investigated. Two tryptophan-binding hydrophobic patches were identified on the conserved face of the GABARAP structure by NMR spectroscopy and molecular docking. GABARAP binding of indole and indole derivatives, including the free amino acid tryptophan was quantified. The two tryptophan binding sites can be clearly distinguished by mapping the NMR spectroscopy-derived residue-specific apparent dissociation constant, K(d), onto the three-dimensional structure of GABARAP. The biological relevance of tryptophan-binding pockets of GABARAP was supported by a highly conserved tryptophan residue in the GABARAP binding region of calreticulin, clathrin heavy chain, and the gamma2 subunit of the GABA(A) receptor. Replacement of tryptophan by alanine abolished ligand binding to GABARAP.

Keyword(s): Adaptor Proteins, Signal Transducing: chemistry (MeSH) ; Adaptor Proteins, Signal Transducing: metabolism (MeSH) ; Amino Acid Sequence (MeSH) ; Animals (MeSH) ; Binding Sites (MeSH) ; Humans (MeSH) ; Hydrophobic and Hydrophilic Interactions (MeSH) ; Indoles: chemistry (MeSH) ; Indoles: metabolism (MeSH) ; Ligands (MeSH) ; Microtubule-Associated Proteins: chemistry (MeSH) ; Microtubule-Associated Proteins: metabolism (MeSH) ; Models, Molecular (MeSH) ; Molecular Sequence Data (MeSH) ; Protein Conformation (MeSH) ; Receptors, GABA-A: metabolism (MeSH) ; Substrate Specificity (MeSH) ; Titrimetry (MeSH) ; Tryptophan: metabolism (MeSH) ; Adaptor Proteins, Signal Transducing ; Indoles ; Ligands ; Microtubule-Associated Proteins ; Receptors, GABA-A ; Tryptophan ; J ; GABARAP (auto) ; indole-binding pocket (auto) ; molecular modeling (auto) ; molecular recognition (auto) ; NMR spectroscopy (auto)

Note: Record converted from VDB: 12.11.2012

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Contributing Institute(s):

1. Molekulare Biophysik (INB-2)

Research Program(s):

1. Funktion und Dysfunktion des Nervensystems (P33)

Appears in the scientific report 2008

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