Journal Article

FZJ-2017-04108

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Mechanism of transmembrane signaling by sensor histidine kinases

Gushchin, I. (Corresponding author) ; Melnikov, I. ; Polovinkin, V. ; Ishchenko, A. ; Yuzhakova, A. ; Buslaev, P. ; Bourenkov, G. ; Grudinin, S. ; Round, E. ; Balandin, T.FZJ* ; Borshchevskiy, V. ; Willbold, D.FZJ* ; Leonard, G. ; Büldt, G. ; Popov, A. ; Gordeliy, V. (Corresponding author)FZJ*

2017
American Association for the Advancement of Science Washington, DC [u.a.]

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Please use a persistent id in citations: doi:10.1126/science.aah6345

Abstract: One of the major and essential classes of transmembrane (TM) receptors, present in all domains of life, is sensor histidine kinases (HKs), parts of two-component signaling systems (TCS). The structural mechanisms of transmembrane signaling by these sensors are poorly understood. We present here crystal structures of the periplasmic sensor domain, the TM domain and the cytoplasmic HAMP domain of the Escherichia coli nitrate/nitrite sensor HK NarQ in the ligand-bound and mutated ligand-free states. The structures reveal that the ligand binding induces significant rearrangements and piston-like shifts of TM helices. The HAMP domain protomers undergo lever-like motions and convert the piston-like motions into helical rotations. Our findings provide the structural framework for complete understanding of TM TCS signaling and for development of antimicrobial treatments targeting TCS.

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Contributing Institute(s):

1. Strukturbiochemie (ICS-6)

Research Program(s):

1. 551 - Functional Macromolecules and Complexes (POF3-551) (POF3-551)

Appears in the scientific report 2017

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Database coverage:
; BIOSIS Previews ; Current Contents - Agriculture, Biology and Environmental Sciences ; Current Contents - Life Sciences ; Current Contents - Physical, Chemical and Earth Sciences ; Ebsco Academic Search ; IF >= 30 ; JCR ; NCBI Molecular Biology Database ; National-Konsortium ; Nationallizenz ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection ; Zoological Record

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