Journal Article

FZJ-2018-03147

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Quantitative proteomics in plant protease substrate identification

Demir, F.FZJ* ; Niedermaier, S.FZJ* ; Villamor, J. G. ; Huesgen, P. (Corresponding author)FZJ*

2018
Wiley-Blackwell Oxford [u.a.]

This record in other databases:      

Please use a persistent id in citations: http://hdl.handle.net/2128/18683  doi:10.1111/nph.14587

Abstract: Proteolysis is a central regulatory mechanism of protein homeostasis and protein function that affects all aspects of plant life. Higher plants encode for hundreds of proteases, but their physiological substrates and hence their molecular functions remain mostly unknown. Current quantitative mass spectrometry‐based proteomics enables unbiased large‐scale interrogation of the proteome and its modifications. Here we provide an overview of proteomics techniques that allow profiling of changes in protein abundance, measurement of proteome turnover rates, identification of protease cleavage sites in vivo and in vitro and determination of protease sequence specificity. We discuss how these techniques can help to reveal protease substrates and determine plant protease function, illustrated by recent studies on selected plant proteases.

---

Contributing Institute(s):

1. Analytik (ZEA-3)

Research Program(s):

1. 582 - Plant Science (POF3-582) (POF3-582)

Appears in the scientific report 2018

---

Database coverage:
; ; BIOSIS Previews ; Current Contents - Agriculture, Biology and Environmental Sciences ; Ebsco Academic Search ; IF >= 5 ; JCR ; NCBI Molecular Biology Database ; National-Konsortium ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection

---