Book/Report

FZJ-2018-04191

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Mikrosonden-Analysen zur Ionenaufnahme in Fichten (Picea abies [L.] Karst.)

Kuhn, A. J. (Corresponding author)FZJ*

1993
Forschungszentrum Jülich GmbH Zentralbibliothek, Verlag Jülich

Please use a persistent id in citations: http://hdl.handle.net/2128/19327

Report No.: Juel-2744

Abstract: The influence of several parameters on the nutrient supply of trees in a forest ecosystem was object of the presented work. Quantitative element analysis of bulk samples was performed by use of inductively coupled plasma optical emission spectroscopy (ICP-OES) and total-reflection X-ray fluorescence spectrometry (TRFA). The main interest was focused on the cellular level by analysing thin sections using the laser-microprobe-mass-analyser (LAMMA) and the energy-dispersive X-ray analyser (EDXA). The nutrient supply was described in 90- to 120-year-old spruce (Picea abies [L .] Karst.) growing in a forest decline stand and was compared to a model-system of two- and three-year-old spruce cultured in different soils. The investigations were focused on: - the element content and the element distribution in mycorrhized spruce roots in relation to soil depth and damage class of the trees analysed - the comparison between the relative element distribution in rnycorrhized roots and in the shoot of spruce cultured in different soils. Stable isotope labelling was performed under defined conditions with spruce trees grown in soil and spruce seedlings from sterile cultures to study the following points concerning the physiological mechanisms of nutrient uptake have been of main interest: - Ion-exchange capacity of mycorrhized roots for Mg, K and Ca in relation to element-concentration, pH and addition of Al - accessibility for Mg, K and Ca to mycorrhized finest roots- temperature dependence of the uptake kinetics for Mg, K and Ca - comparison of ion-uptake in sterile and mycorrhized spruce seedlings - balance of ion-uptake in spruce cultured in soil and the influence of Al-addition. $\textbf{Nutrient status of old spruce and spruce cultured in soli:}$ Two old spruce trees of differing vitality(stand "POSTTURM"), forestry commission Farchau / Ratzeburg ; damage class 0 and 3, respectively) were analysed. Finest roots as well as the surrounding soil were collected in 3 soil depths (0-10crn, 10-30cm, 30-50cm). The element distribution within the mycorrhizas determined with LAMMA and the element content of the mycorrhizas and of the centrifuged soil solutions were measured by use of ICP-OES. There were no clear correlations between element distribution, element content of mycorrhizas and element content of soil solutions. The Al content of mycorrhizas rises with increasing soil depth. The element distribution clearly shows higher Al contents in the cortex than in the walls of the primary xylem. In all samples the Mg content is clearly higher in the lumen of cortical cells than in the corresponding cell walls. The distribution of Ca is reverse. Mycorrhizas from trees of damage class 3 collected in 30-50cm soil-depth show lower element contents of Na, Mg, K and Ca relative to those from trees of damage class 0 grown in the same depth. The largest difference in the element distribution in mycorrhizas from soil depth are manifested as higher Na- and lower K-contents over the entire cross-section and as lower Mg content in the lumina of cortex cells at damage class O.

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Contributing Institute(s):

1. Publikationen vor 2000 (PRE-2000)

Research Program(s):

1. 899 - ohne Topic (POF3-899) (POF3-899)

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