Journal Article

FZJ-2018-06216

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Chip-Based Heat Stimulation for Modulating Signal Propagation in HL-1 Cell Networks

Dang, K. M. ; Rinklin, P. ; Afanasenkau, D. ; Westmeyer, G. ; Schürholz, T. ; Wiegand, S.FZJ* ; Wolfrum, B. (Corresponding author)FZJ*

2018
Wiley-VCH Weinheim

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Please use a persistent id in citations: doi:10.1002/adbi.201800138

Abstract: Signal propagation in cardiac cell networks can be modulated by heat stimulation. Here, we investigate the response of a connected HL-1 cardiomyocyte cell network to the application of confined heat stimuli using Ca2+ imaging. Localized temperature gradients are generated by resistive heating via microwire arrays on a chip surface, which serves as a substrate for growing a confluent cell network. We demonstrate that upon heat stimulation, the velocity of the propagating Ca2+ wave in the network is locally increased, leading to a deformation of the wavefront. Furthermore, we show evidence of a change in the signal propagation direction caused by a relocation of the pacemaker cell. This effect might be used in future applications, where heat is employed as an alternative modality for cell stimulation protocols.

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Contributing Institute(s):

1. Weiche Materie (ICS-3)

Research Program(s):

1. 551 - Functional Macromolecules and Complexes (POF3-551) (POF3-551)

Appears in the scientific report 2018

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