TY  - JOUR
AU  - Achtsnicht, Stefan
AU  - Tödter, Julia
AU  - Niehues, Julia
AU  - Telöken, Matthias
AU  - Offenhäusser, Andreas
AU  - Krause, Hans-Joachim
AU  - Schröper, Florian
TI  - 3D Printed Modular Immunofiltration Columns for Frequency Mixing-Based Multiplex Magnetic Immunodetection
JO  - Sensors
VL  - 19
IS  - 1
SN  - 1424-8220
CY  - Basel
PB  - MDPI
M1  - FZJ-2019-00067
SP  - 148 -
PY  - 2019
AB  - For performing point-of-care molecular diagnostics, magnetic immunoassays constitute a promising alternative to established enzyme-linked immunosorbent assays (ELISA) because they are fast, robust and sensitive. Simultaneous detection of multiple biomolecular targets from one body fluid sample is desired. The aim of this work is to show that multiplex magnetic immunodetection based on magnetic frequency mixing by means of modular immunofiltration columns prepared for different targets is feasible. By calculations of the magnetic response signal, the required spacing between the modules was determined. Immunofiltration columns were manufactured by 3D printing and antibody immobilization was performed in a batch approach. It was shown experimentally that two different target molecules in a sample solution could be individually detected in a single assaying step with magnetic measurements of the corresponding immobilization filters. The arrangement order of the filters and of a negative control did not influence the results. Thus, a simple and reliable approach to multi-target magnetic immunodetection was demonstrated.
LB  - PUB:(DE-HGF)16
C6  - pmid:30609859
UR  - <Go to ISI:>//WOS:000458574600148
DO  - DOI:10.3390/s19010148
UR  - https://juser.fz-juelich.de/record/859131
ER  -