Journal Article

FZJ-2019-00067

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png 3D Printed Modular Immunofiltration Columns for Frequency Mixing-Based Multiplex Magnetic Immunodetection

Achtsnicht, S.FZJ* ; Tödter, J. ; Niehues, J. ; Telöken, M.FZJ* ; Offenhäusser, A.FZJ* ; Krause, H.-J. (Corresponding author)FZJ* ; Schröper, F. (Corresponding author)

2019
MDPI Basel

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Please use a persistent id in citations: http://hdl.handle.net/2128/21646  doi:10.3390/s19010148

Abstract: For performing point-of-care molecular diagnostics, magnetic immunoassays constitute a promising alternative to established enzyme-linked immunosorbent assays (ELISA) because they are fast, robust and sensitive. Simultaneous detection of multiple biomolecular targets from one body fluid sample is desired. The aim of this work is to show that multiplex magnetic immunodetection based on magnetic frequency mixing by means of modular immunofiltration columns prepared for different targets is feasible. By calculations of the magnetic response signal, the required spacing between the modules was determined. Immunofiltration columns were manufactured by 3D printing and antibody immobilization was performed in a batch approach. It was shown experimentally that two different target molecules in a sample solution could be individually detected in a single assaying step with magnetic measurements of the corresponding immobilization filters. The arrangement order of the filters and of a negative control did not influence the results. Thus, a simple and reliable approach to multi-target magnetic immunodetection was demonstrated.

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Contributing Institute(s):

1. Bioelektronik (ICS-8)

Research Program(s):

1. 553 - Physical Basis of Diseases (POF3-553) (POF3-553)
2. 523 - Controlling Configuration-Based Phenomena (POF3-523) (POF3-523)

Appears in the scientific report 2019

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Database coverage:
; ; ; ; Clarivate Analytics Master Journal List ; DOAJ Seal ; Ebsco Academic Search ; IF < 5 ; JCR ; NCBI Molecular Biology Database ; PubMed Central ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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