TY  - JOUR
AU  - Achtsnicht, Stefan
AU  - Neuendorf, Christian
AU  - Fassbender, Tobias
AU  - Nölke, Greta
AU  - Offenhäusser, Andreas
AU  - Krause, Hans-Joachim
AU  - Schröper, Florian
TI  - Sensitive and rapid detection of cholera toxin subunit B using magnetic frequency mixing detection
JO  - PLOS ONE
VL  - 14
IS  - 7
SN  - 1932-6203
CY  - San Francisco, California, US
PB  - PLOS
M1  - FZJ-2019-03843
SP  - e0219356 -
PY  - 2019
AB  - Cholera is a life-threatening disease caused by the cholera toxin (CT) as produced by some Vibrio cholerae serogroups. In this research we present a method which directly detects the toxin’s B subunit (CTB) in drinking water. For this purpose we performed a magnetic sandwich immunoassay inside a 3D immunofiltration column. We used two different commercially available antibodies to capture CTB and for binding to superparamagnetic beads. ELISA experiments were performed to select the antibody combination. The beads act as labels for the magnetic frequency mixing detection technique. We show that the limit of detection depends on the type of magnetic beads. A nonlinear Hill curve was fitted to the calibration measurements by means of a custom-written python software. We achieved a sensitive and rapid detection of CTB within a broad concentration range from 0.2 ng/ml to more than 700 ng/ml.
LB  - PUB:(DE-HGF)16
C6  - pmid:31276546
UR  - <Go to ISI:>//WOS:000484936300069
DO  - DOI:10.1371/journal.pone.0219356
UR  - https://juser.fz-juelich.de/record/863872
ER  -