Journal Article

FZJ-2019-03843

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Sensitive and rapid detection of cholera toxin subunit B using magnetic frequency mixing detection

Achtsnicht, S.FZJ* ; Neuendorf, C. ; Fassbender, T.FZJ* ; Nölke, G. ; Offenhäusser, A.FZJ* ; Krause, H.-J. (Corresponding author)FZJ* ; Schröper, F. (Corresponding author)

2019
PLOS San Francisco, California, US

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Please use a persistent id in citations: http://hdl.handle.net/2128/22675  doi:10.1371/journal.pone.0219356

Abstract: Cholera is a life-threatening disease caused by the cholera toxin (CT) as produced by some Vibrio cholerae serogroups. In this research we present a method which directly detects the toxin’s B subunit (CTB) in drinking water. For this purpose we performed a magnetic sandwich immunoassay inside a 3D immunofiltration column. We used two different commercially available antibodies to capture CTB and for binding to superparamagnetic beads. ELISA experiments were performed to select the antibody combination. The beads act as labels for the magnetic frequency mixing detection technique. We show that the limit of detection depends on the type of magnetic beads. A nonlinear Hill curve was fitted to the calibration measurements by means of a custom-written python software. We achieved a sensitive and rapid detection of CTB within a broad concentration range from 0.2 ng/ml to more than 700 ng/ml.

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Contributing Institute(s):

1. Bioelektronik (ICS-8)

Research Program(s):

1. 523 - Controlling Configuration-Based Phenomena (POF3-523) (POF3-523)

Appears in the scientific report 2019

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