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@ARTICLE{Achtsnicht:863872,
      author       = {Achtsnicht, Stefan and Neuendorf, Christian and Fassbender,
                      Tobias and Nölke, Greta and Offenhäusser, Andreas and
                      Krause, Hans-Joachim and Schröper, Florian},
      title        = {{S}ensitive and rapid detection of cholera toxin subunit
                      {B} using magnetic frequency mixing detection},
      journal      = {PLOS ONE},
      volume       = {14},
      number       = {7},
      issn         = {1932-6203},
      address      = {San Francisco, California, US},
      publisher    = {PLOS},
      reportid     = {FZJ-2019-03843},
      pages        = {e0219356 -},
      year         = {2019},
      abstract     = {Cholera is a life-threatening disease caused by the cholera
                      toxin (CT) as produced by some Vibrio cholerae serogroups.
                      In this research we present a method which directly detects
                      the toxin’s B subunit (CTB) in drinking water. For this
                      purpose we performed a magnetic sandwich immunoassay inside
                      a 3D immunofiltration column. We used two different
                      commercially available antibodies to capture CTB and for
                      binding to superparamagnetic beads. ELISA experiments were
                      performed to select the antibody combination. The beads act
                      as labels for the magnetic frequency mixing detection
                      technique. We show that the limit of detection depends on
                      the type of magnetic beads. A nonlinear Hill curve was
                      fitted to the calibration measurements by means of a
                      custom-written python software. We achieved a sensitive and
                      rapid detection of CTB within a broad concentration range
                      from 0.2 ng/ml to more than 700 ng/ml.},
      cin          = {ICS-8},
      ddc          = {610},
      cid          = {I:(DE-Juel1)ICS-8-20110106},
      pnm          = {523 - Controlling Configuration-Based Phenomena (POF3-523)},
      pid          = {G:(DE-HGF)POF3-523},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:31276546},
      UT           = {WOS:000484936300069},
      doi          = {10.1371/journal.pone.0219356},
      url          = {https://juser.fz-juelich.de/record/863872},
}