Journal Article

FZJ-2020-00321

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Neutron crystallographic study of heterotrimeric glutamine amidotransferase CAB

Li, L. ; Adachi, M. ; Yu, J. ; Kato, K. ; Shinoda, A. ; Ostermann, A. ; Schrader, T. E.FZJ* ; Ose, T. ; Yao, M. (Corresponding author)

2019
Blackwell Oxford [u.a.]

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Please use a persistent id in citations: http://hdl.handle.net/2128/24007  doi:10.1107/S2053230X19000220

Abstract: Heterotrimeric glutamine amidotransferase CAB (GatCAB) possesses anammonia-self-sufficient mechanism in which ammonia is produced and usedin the inner complex by GatA and GatB, respectively. The X-ray structure ofGatCAB revealed that the two identified active sites of GatA and GatB aremarkedly distant, but are connected in the complex by a channel of 30 A ˚ inlength. In order to clarify whether ammonia is transferred through this channelin GatCAB by visualizing ammonia, neutron diffraction studies are indispensable.Here, GatCAB crystals were grown to approximate dimensions of2.8 0.8 0.8 mm (a volume of 1.8 mm3) with the aid of a polymer usingmicroseeding and macroseeding processes. Monochromatic neutron diffractiondata were collected using the neutron single-crystal diffractometer BIODIFF atthe Heinz Maier-Leibnitz Zentrum, Germany. The GatCAB crystals belonged tospace group P212121, with unit-cell parameters a = 74.6, b = 94.5, c = 182.5 A ˚ andwith one GatCAB complex (molecular mass 119 kDa) in the asymmetric unit.This study represented a challenge in current neutron diffraction technology.

Keyword(s): Health and Life (1st) ; Biology (2nd)

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Contributing Institute(s):

1. JCNS-FRM-II (JCNS-FRM-II)
2. Neutronenstreuung (JCNS-1)
3. Heinz Maier-Leibnitz Zentrum (MLZ)

Research Program(s):

1. 6G4 - Jülich Centre for Neutron Research (JCNS) (POF3-623) (POF3-623)
2. 6215 - Soft Matter, Health and Life Sciences (POF3-621) (POF3-621)
3. 6G15 - FRM II / MLZ (POF3-6G15) (POF3-6G15)

Experiment(s):

1. BIODIFF: Diffractometer for large unit cells (NL1)

Appears in the scientific report 2019

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Database coverage:
; ; BIOSIS Previews ; Clarivate Analytics Master Journal List ; Ebsco Academic Search ; IF < 5 ; JCR ; NCBI Molecular Biology Database ; PubMed Central ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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