Journal Article

FZJ-2020-02607

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Permutation testing of Fourier shell correlation for resolution estimation of cryo-EM maps

Beckers, M. (Corresponding author)FZJ* ; Sachse, C. (Corresponding author)FZJ*

2020
Elsevier San Diego, Calif.

This record in other databases:      

Please use a persistent id in citations: http://hdl.handle.net/2128/25483  doi:10.1016/j.jsb.2020.107579

Abstract: Fourier shell correlation (FSC) has become a standard quantity for resolution estimation in electron cryo-microscopy. However, the resolution determination step is still subjective and not fully automated as it involves a series of map interventions before FSC computation and includes the selection of a common threshold. Here, we apply the statistical methods of permutation testing and false discovery rate (FDR) control to the resolution-dependent correlation measure. The approach allows fully automated and mask-free resolution determination based on statistical thresholding of FSC curves. We demonstrate the applicability for global, local and directional resolution estimation and show that the developed criterion termed FDR-FSC gives realistic resolution estimates based on statistical significance while eliminating the need of any map manipulations. The algorithms are implemented in a user-friendly GUI based software tool termed SPoC (https://github.com/MaximilianBeckers/SPOC).

---

Contributing Institute(s):

1. Strukturbiologie (ER-C-3)

Research Program(s):

1. 551 - Functional Macromolecules and Complexes (POF3-551) (POF3-551)

Appears in the scientific report 2020

---

Database coverage:
; ; ; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; Ebsco Academic Search ; Essential Science Indicators ; IF < 5 ; JCR ; NCBI Molecular Biology Database ; Nationallizenz ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Web of Science Core Collection ; Zoological Record

---