Journal Article/Contribution to a conference proceedings

FZJ-2020-04216

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png In Situ Dynamic Light Scattering Complementing Neutron Spin Echo Measurements on Protein Samples

Balacescu, L.FZJ* ; Vögl, F.FZJ* ; Staringer, S.FZJ* ; Ossovyi, V.FZJ* ; Brandl, G.FZJ* ; Lumma, N. ; Feilbach, H.FZJ* ; Holderer, O.FZJ* ; Pasini, S.FZJ* ; Stadler, A.FZJ* ; Fitter, J.FZJ* ; Schrader, T. E. (Corresponding author)FZJ*

2020
Springer Science+Business Media Berlin

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Please use a persistent id in citations: http://hdl.handle.net/2128/26042  doi:10.1134/S1027451020070046

Abstract: Monitoring the state of the sample on the minute-time scale is crucial in case of sensitive soft matter or biological samples, given that neutron spin echo measurements take up to several days. Moreover, there is no method to interpret the normalized intermediate scattering function obtained by neutron spin echo measurements if relevant sample properties change during the measurement. Dynamic light scattering provides information on the diffusion constant of particles in solution (biological macromolecules like proteins, protein aggregates, polymer particles, etc.) with average hydrodynamic radii in a broad range from a few nanometers up to several microns. This information can be obtained within a few minutes and it offers a good overview of the current sample state. Details on the novel in situ dynamic light scattering set-up with one fixed scattering angle and first results obtained on a molten globule state of apo-myoglobin are presented.

Keyword(s): Instrument and Method Development (1st) ; Instrument and Method Development (2nd)

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Contributing Institute(s):

1. JCNS-FRM-II (JCNS-FRM-II)
2. Neutronenstreuung (JCNS-1)
3. Heinz Maier-Leibnitz Zentrum (MLZ)
4. Zelluläre Strukturbiologie (IBI-6)

Research Program(s):

1. 6G4 - Jülich Centre for Neutron Research (JCNS) (POF3-623) (POF3-623)
2. 6G15 - FRM II / MLZ (POF3-6G15) (POF3-6G15)
3. 6215 - Soft Matter, Health and Life Sciences (POF3-621) (POF3-621)

Experiment(s):

1. J-NSE: Neutron spin-echo spectrometer (NL2ao)

Appears in the scientific report 2020

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Database coverage:
; Clarivate Analytics Master Journal List ; DEAL Springer ; Emerging Sources Citation Index ; SCOPUS ; Web of Science Core Collection

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