Journal Article

FZJ-2020-05382

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Assembly of Multi‐Spheroid Cellular Architectures by Programmable Droplet Merging

Cui, H. ; Wang, X. ; Wesslowski, J. ; Tronser, T. ; Rosenbauer, J.FZJ* ; Schug, A.FZJ* ; Davidson, G. ; Popova, A. A. ; Levkin, P. A. (Corresponding author)

2021
Wiley-VCH Weinheim

This record in other databases:      

Please use a persistent id in citations: http://hdl.handle.net/2128/27125  doi:10.1002/adma.202006434

Abstract: Artificial multicellular systems are gaining importance in the field of tissue engineering and regenerative medicine. Reconstruction of complex tissue architectures in vitro is nevertheless challenging, and methods permitting controllable and high‐throughput fabrication of complex multicellular architectures are needed. Here, a facile and high‐throughput method is developed based on a tunable droplet‐fusion technique, allowing programmed assembly of multiple cell spheroids into complex multicellular architectures. The droplet‐fusion technique allows for construction of various multicellular architectures (double‐spheroids, multi‐spheroids, hetero‐spheroids) in a miniaturized high‐density array format. As an example of application, the propagation of Wnt signaling is investigated within hetero‐spheroids formed from two fused Wnt‐releasing and Wnt‐reporter cell spheroids. The developed method provides an approach for miniaturized, high‐throughput construction of complex 3D multicellular architectures and can be applied for studying various biological processes including cell signaling, cancer invasion, embryogenesis, and neural development.

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Contributing Institute(s):

1. Jülich Supercomputing Center (JSC)
2. John von Neumann - Institut für Computing (NIC)

Research Program(s):

1. 5111 - Domain-Specific Simulation & Data Life Cycle Labs (SDLs) and Research Groups (POF4-511) (POF4-511)
2. Forschergruppe Schug (hkf6_20200501) (hkf6_20200501)

Appears in the scientific report 2021

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Database coverage:
; ; ; Clarivate Analytics Master Journal List ; Current Contents - Engineering, Computing and Technology ; Current Contents - Physical, Chemical and Earth Sciences ; DEAL Wiley ; Essential Science Indicators ; IF >= 25 ; JCR ; Nationallizenz ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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