Journal Article

FZJ-2021-00020

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Size Measurement of Extracellular Vesicles and Synthetic Liposomes: The Impact of the Hydration Shell and the Protein Corona

Varga, Z. (Corresponding author)Extern* ; Fehér, B. ; Kitka, D. ; Wacha, A. ; Bóta, A. ; Berényi, S. ; Pipich, V.FZJ* ; Fraikin, J.-L.

2020
Science Direct [S.l.]

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Please use a persistent id in citations: http://hdl.handle.net/2128/26880  doi:10.1016/j.colsurfb.2020.111053

Abstract: Size characterization of extracellular vesicles (EVs) and drug delivery liposomes is of great importance in their applications in diagnosis and therapy of diseases. There are many different size characterization techniques used in the field, which often report different size values. Besides technological biases, these differences originate from the fact that various methods measure different physical quantities to determine particle size. In this study, the size of synthetic liposomes with nominal diameters of 50nm and 100nm, and red blood cell-derived EVs (REVs) were measured with established optical methods, such as dynamic light scattering (DLS) and nanoparticle tracking analysis (NTA), and with emerging non-optical methods such as microfluidic resistive pulse sensing (MRPS) and very small-angle neutron scattering (VSANS). The comparison of the hydrodynamic sizes obtained by DLS and NTA with the sizes corresponding to the excluded volume of the particles by MRPS enabled the estimation of the thickness of the hydration shell of the particles. The comparison of diameter values corresponding to the boundary of the phospholipid bilayer obtained from VSANS measurements with MRPS size values revealed the thickness of the polyethylene glycol-layer in case of synthetic liposomes, and the thickness of the protein corona in case of REVs.

Keyword(s): Health and Life (1st) ; Biology (2nd)

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Contributing Institute(s):

1. Neutronenstreuung (JCNS-1)
2. JCNS-4 (JCNS-4)
3. JCNS-FRM-II (JCNS-FRM-II)
4. Heinz Maier-Leibnitz Zentrum (MLZ)

Research Program(s):

1. 6215 - Soft Matter, Health and Life Sciences (POF3-621) (POF3-621)
2. 6G4 - Jülich Centre for Neutron Research (JCNS) (POF3-623) (POF3-623)
3. 6G15 - FRM II / MLZ (POF3-6G15) (POF3-6G15)

Experiment(s):

1. KWS-3: Very small angle scattering diffractometer with focusing mirror (NL3auS)

Appears in the scientific report 2020

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Database coverage:
; ; ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; Ebsco Academic Search ; Essential Science Indicators ; IF < 5 ; JCR ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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