Journal Article

FZJ-2021-01307

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Stretchable electrical cell-substrate impedance sensor platform for monitoring cell monolayers under strain

Zhou, C. (Corresponding author) ; Bette, S. ; Babendreyer, A. ; Hoffmann, C.FZJ* ; Gerlach, S.FZJ* ; Kremers, T. ; Ludwig, A. ; Hoffmann, B.FZJ* ; Merkel, R.FZJ* ; Uhlig, S. ; Schnakenberg, U.

2021
Elsevier Science Amsterdam [u.a.]

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Please use a persistent id in citations: http://hdl.handle.net/2128/27526  doi:10.1016/j.snb.2021.129656

Abstract: Stretchable microelectrodes paired with an ultra-elastic substrate can be used for electrical sensing of mechanically stretched cells and cell monolayers. Here, we present the development of a cell-stretching platform with thin-film interdigitated microelectrodes. Up to 35 % cyclic stretch are feasible with a novel interlaced meander design connected to the microelectrodes and using Poly(dimethylsiloxane) (PDMS) with a Young’s modulus of 50 kPa as an ultra-elastic substrate. Reliable electrical contacting of the microelectrodes under stretch was achieved by perforation of the contact pads. The novel platform enables label-free, real-time electrical cell-substrate impedance (ECIS) monitoring of cell monolayers. Proof-of-concept experiments indicated that electrical impedance of Madin-Darby canine kidney (MDCK) cell monolayers increased sharply by uniaxial mechanical strain above 20 %. For comparison, human alveolar basal epithelial adenocarcinoma (A549) cell monolayers, which are known to lack mature cell junctions, showed a continuous decrease of electrical impedance over the whole applied strain range of 35 %. The data reveal that impedance changes upon stretching depend on epithelial cell types and existence of tight cellular junctions. The system provides the basis for reliable continuous long-term monitoring of electrical properties of cell monolayers under strain by electrical impedance spectroscopy, e.g., to monitor epithelial permeability changes in real time and under label-free conditions to screen the influence of pharmacological substances.

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Contributing Institute(s):

1. Mechanobiologie (IBI-2)

Research Program(s):

1. 311 - Zellbiologie und Tumorbiologie (POF4-311) (POF4-311)
2. DFG project 273723265 - Mechanosensation und Mechanoreaktion in epidermalen Systemen (273723265) (273723265)
3. 5243 - Information Processing in Distributed Systems (POF4-524) (POF4-524)

Appears in the scientific report 2021

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Database coverage:
; ; ; Clarivate Analytics Master Journal List ; Current Contents - Engineering, Computing and Technology ; Current Contents - Physical, Chemical and Earth Sciences ; Ebsco Academic Search ; Essential Science Indicators ; IF >= 5 ; JCR ; Nationallizenz ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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