Journal Article

FZJ-2021-05912

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Light-Addressable Actuator-Sensor Platform for Monitoring and Manipulation of pH Gradients in Microfluidics: A Case Study with the Enzyme Penicillinase

Welden, R. ; Jablonski, M. ; Wege, C. ; Keusgen, M. ; Wagner, P. H. ; Wagner, T. ; Schöning, M. J. (Corresponding author)FZJ*

2021
MDPI Basel

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Please use a persistent id in citations: http://hdl.handle.net/2128/33095  doi:10.3390/bios11060171

Abstract: The feasibility of light-addressed detection and manipulation of pH gradients inside an electrochemical microfluidic cell was studied. Local pH changes, induced by a light-addressable electrode (LAE), were detected using a light-addressable potentiometric sensor (LAPS) with different measurement modes representing an actuator-sensor system. Biosensor functionality was examined depending on locally induced pH gradients with the help of the model enzyme penicillinase, which had been immobilized in the microfluidic channel. The surface morphology of the LAE and enzyme-functionalized LAPS was studied by scanning electron microscopy. Furthermore, the penicillin sensitivity of the LAPS inside the microfluidic channel was determined with regard to the analyte’s pH influence on the enzymatic reaction rate. In a final experiment, the LAE-controlled pH inhibition of the enzyme activity was monitored by the LAPS

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Contributing Institute(s):

1. Bioelektronik (IBI-3)

Research Program(s):

1. 5241 - Molecular Information Processing in Cellular Systems (POF4-524) (POF4-524)

Appears in the scientific report 2022

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