Journal Article

FZJ-2021-06056

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Numaswitch: an efficient high-titer expression platform to produce peptides and small proteins

Nguyen, B.-N. ; Tieves, F. ; Rohr, T. ; Wobst, H. ; Schöpf, F. S. ; Solano, J. D. M. ; Schneider, J.FZJ* ; Stock, J. ; Uhde, A. ; Kalthoff, T. ; Jaeger, K. E.FZJ* ; Schmitt, L. ; Schwarz, C. (Corresponding author)Extern*

2021
Springer Heidelberg

This record in other databases:      

Please use a persistent id in citations: http://hdl.handle.net/2128/30461  doi:10.1186/s13568-021-01204-w

Abstract: The production of peptides as active pharmaceutical ingredients (APIs) by recombinant technologies is of emerging interest. A reliable production platform, however, is still missing due the inherent characteristics of peptides such as proteolytic sensitivity, aggregation and cytotoxicity. We have developed a new technology named Numaswitch solving present limitations. Numaswitch was successfully employed for the production of diverse peptides and small proteins varying in length, physicochemical and functional characteristics, including Teriparatide, Linaclotide, human β-amyloid and Serum amyloid A3. Additionally, the potential of Numaswitch for a cost-efficient commercial production is demonstrated yielding > 2 g Teriparatide per liter fermentation broth in a quality meeting API standard.

---

Contributing Institute(s):

1. Biotechnologie (IBG-1)
2. Institut für Molekulare Enzymtechnologie (HHUD) (IMET)

Research Program(s):

1. 2172 - Utilization of renewable carbon and energy sources and engineering of ecosystem functions (POF4-217) (POF4-217)

Appears in the scientific report 2021

---

Database coverage:
; ; ; ; Article Processing Charges ; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; DOAJ Seal ; Ebsco Academic Search ; Essential Science Indicators ; Fees ; IF < 5 ; JCR ; PubMed Central ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

---