Journal Article

FZJ-2022-02987

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Structural basis for the inhibition of IAPP fibril formation by the co-chaperonin prefoldin.

Törner, R. ; Kupreichyk, T.FZJ* ; Gremer, L.FZJ* ; Debled, E. C. ; Fenel, D. ; Schemmert, S.FZJ* ; Gans, P. ; Willbold, D.FZJ* ; Schoehn, G. ; Hoyer, W. (Corresponding author)FZJ* ; Boisbouvier, J. (Corresponding author)

2022
Nature Publishing Group UK [London]

This record in other databases:      

Please use a persistent id in citations: http://hdl.handle.net/2128/31660  doi:10.1038/s41467-022-30042-y

Abstract: Chaperones, as modulators of protein conformational states, are key cellular actors to prevent the accumulation of fibrillar aggregates. Here, we integrated kinetic investigations with structural studies to elucidate how the ubiquitous co-chaperonin prefoldin inhibits diabetes associated islet amyloid polypeptide (IAPP) fibril formation. We demonstrated that both human and archaeal prefoldin interfere similarly with the IAPP fibril elongation and secondary nucleation pathways. Using archaeal prefoldin model, we combined nuclear magnetic resonance spectroscopy with electron microscopy to establish that the inhibition of fibril formation is mediated by the binding of prefoldin's coiled-coil helices to the flexible IAPP N-terminal segment accessible on the fibril surface and fibril ends. Atomic force microscopy demonstrates that binding of prefoldin to IAPP leads to the formation of lower amounts of aggregates, composed of shorter fibrils, clustered together. Linking structural models with observed fibrillation inhibition processes opens perspectives for understanding the interference between natural chaperones and formation of disease-associated amyloids.

Keyword(s): Amyloid: metabolism (MeSH) ; Chaperonins (MeSH) ; Humans (MeSH) ; Islet Amyloid Polypeptide (MeSH) ; Molecular Chaperones: metabolism (MeSH) ; Amyloid ; Islet Amyloid Polypeptide ; Molecular Chaperones ; prefoldin ; Chaperonins

---

Contributing Institute(s):

1. Strukturbiochemie (IBI-7)

Research Program(s):

1. 5244 - Information Processing in Neuronal Networks (POF4-524) (POF4-524)
2. BETACONTROL - Control of amyloid formation via beta-hairpin molecular recognition features (726368) (726368)

Appears in the scientific report 2022

---

Database coverage:
; ; ; ; Article Processing Charges ; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Current Contents - Agriculture, Biology and Environmental Sciences ; Current Contents - Life Sciences ; Current Contents - Physical, Chemical and Earth Sciences ; DOAJ Seal ; Essential Science Indicators ; Fees ; IF >= 15 ; JCR ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection ; Zoological Record

---