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| Home > Publications database > Culture optimization and characterization of three Porphyridium strains for valuable compound production |
| Bachelor Thesis | FZJ-2023-00615 |
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2022
Please use a persistent id in citations: http://hdl.handle.net/2128/33598
Abstract: The optimal conditions to maximize phycoerythrin- (PE) and exopolysaccharide (EPS) production in the three photoautotrophic red marine microalgal strains of Porphyridium purpureum (SAG 1380-1a/-1d) and cruentum (UTEX 161) were researched. Design of experiment software (DoE) was used in planning and evaluation. Dependencies were found between light intensity, nitrogen and magnesium concentrations and the PE and EPS productivities. The maximal PE productivity (4,96 µg mL-1d-1) was found in P. purpureum SAG 1380-1d. Contrasting, the highest EPS productivity (5,75 g l-1 d-1) was found in P. purpureum SAG 1380-1a. However, optimization efforts in EPS production remained low due to low dependencies under tested growth conditions. The optimal product inducing conditions for all strains were identified at ~40 µmol photon m-2 s-1 and 9-15 mM nitrogen. The optimal magnesium concentration ranged between 5, 38 and 45 mM for UTEX 161, SAG 1380-1a and -1d, respectively. The pilot scale cultivation under greenhouse conditions could not be finished, because of temperature-stress during the cultivation.
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