Journal Article

FZJ-2023-03103

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Delayed cytotoxic and genotoxic effects in a human cell line following X-irradiation

Kriehuber, R. (Corresponding author)FZJ* ; Simko, M. ; Schiffmann, D. ; Trott, K.-R.

1999
Informa Healthcare Abingdon

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Please use a persistent id in citations: doi:10.1080/095530099139782

Abstract: Background: In order to clarify the relationship between delayed reproductive death and radiation-induced genomic instability, the colony-forming efficiency of surviving, irradiated human squamous carcinoma cells and centromere positive as well as centromere negative micronuclei in surviving progeny were examined.Materials and methods: Colony-forming ability and micronucleus (MN) frequency in binucleated cells 24 h after the addition of cytochalasin B during 2 weeks of post-irradiation growth were determined in a squamous cell carcinoma cell line (SCL-II) of human origin. In addition, centromeres in micronuclei were detected using FISH.Results: In the human epithelial cell line used for these experiments, delayed reproductive death was pronounced and persisted for at least 2 weeks after irradiation. Although there is evidence for an increased rate of centromere positive micronuclei, but not of centromere negative micronuclei, arising during the first week of post-irradiation proliferation, this decreases later while the rate of delayed reproductive death remains elevated.Conclusion: In the studied cell line, the observed delayed reproductive death is not closely related to the investigated criteria of radiation-induced genomic instability. This casts doubt on the common assumption that delayed reproductive death is a direct manifestation of radiation-induced genomic instability.

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Contributing Institute(s):

1. Sicherheit und Strahlenschutz, Umgebungsüberwachung,Strahlenbiologie (S-US)

Research Program(s):

1. 899 - ohne Topic (POF4-899) (POF4-899)

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