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| Home > Publications database > Characterization of Chlorellales cell walls – Spatio-temporal refined biochemical and morphological characterization of Chlorella sorokinianaand vulgaris, and Parachlorella kessleri |
| Home > Publications database > Characterization of Chlorellales cell walls – Spatio-temporal refined biochemical and morphological characterization of Chlorella sorokinianaand vulgaris, and Parachlorella kessleri |
| Master Thesis | FZJ-2023-05047 |
; ;
2021
Fachhochschule Aachen
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Please use a persistent id in citations: doi:10.34734/FZJ-2023-05047
Abstract: Microalgae of the family Chlorellaceae are of special interest to the food, feed, and nutraceutical industry, due to their high content of valuable nutrients. However, the bioaccessibility is limited by the rigid and polymorph cell wall of these species. This work aimed to characterize the cell walls of three Chlorellaceae strains in-vivo and in-vitro under industry-related culturing conditions. Histochemical staining and detailed analyses of cell wall monosaccharide composition identified changes in the composition during cell maturation in Chlorella sorokiniana SAG 211-8k, Chlorella vulgaris SAG 211-11b and Parachlorella kessleri SAG 211-11g. The cell walls of C. sorokiniana and P. kessleri developed a higher hemicellulose content (defined as susceptible to 2 mol/L trifluoroacetic acid hydrolysis) with stable amounts of the recalcitrance components (susceptible to 6 mol/L HCl). In contrast, the cell wall of C. vulgaris incorporated increasingly recalcitrance components and developed higher robustness.Additionally, a novel endogenous cell-wall-lytic enzyme (autologous Autolysin) was extracted from C. sorokiniana and P. kessleri. It showed endo-β-1,3-Glucanase activity, and partial autologous cell wall degradation in both strains. Thus, presenting a first step towards more effective methods for increased bioaccessibility, protoplast formation and genetic manipulation of Chlorellaceae strains.
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