Journal Article

FZJ-2024-01015

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Baculovirus Actin Rearrangement-Inducing Factor 1 Can Remodel the Mammalian Actin Cytoskeleton

Steffen, A. ; Reusch, B. ; Gruteser, N. ; Mainz, D. ; Roncarati, R. ; Baumann, A.FZJ* ; Stradal, T. E. B. ; Knebel-Mörsdorf, D. (Corresponding author)

2023
ASM Birmingham, Ala.

This record in other databases:      

Please use a persistent id in citations: doi:10.1128/spectrum.05189-22  doi:10.34734/FZJ-2024-01015

Abstract: The actin rearrangement-inducing factor 1 (Arif-1) of Autographa californicamultiple nucleopolyhedrovirus (AcMNPV) is an early viral protein that manipulates theactin cytoskeleton of host insect cells. Arif-1 is conserved among alphabaculoviruses andis responsible for the accumulation of F-actin at the plasma membrane during the earlyphase of infection. However, the molecular mechanism underlying Arif-1-induced corticalactin accumulation is still open. Recent studies have demonstrated the formation ofinvadosome-like structures induced by Arif-1, suggesting a function in systemic virusspread. Here, we addressed whether Arif-1 is able to manipulate the actin cytoskeletonof mammalian cells comparably to insect cells. Strikingly, transient overexpression ofArif-1 in B16-F1 mouse melanoma cells revealed pronounced F-actin remodeling. Actinassembly was increased, and intense membrane ruffling occurred at the expense of substrate-associated lamellipodia. Deletion mutagenesis studies of Arif-1 confirmed that theC-terminal cytoplasmic region was not sufficient to induce F-actin remodeling, supportingthat the transmembrane region for Arif-1 function is also required in mammaliancells. The similarities between Arif-1-induced actin remodeling in insect and mammaliancells indicate that Arif-1 function relies on conserved cellular interaction partners and signaltransduction pathways, thus providing an experimental tool to elucidate the underlyingmechanism.

---

Contributing Institute(s):

1. Molekular- und Zellphysiologie (IBI-1)

Research Program(s):

1. 5244 - Information Processing in Neuronal Networks (POF4-524) (POF4-524)

Appears in the scientific report 2024

---

Database coverage:
; ; ; ; Article Processing Charges ; BIOSIS Previews ; BIOSIS Reviews Reports And Meetings ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; DOAJ Seal ; Essential Science Indicators ; Fees ; IF < 5 ; JCR ; PubMed Central ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

---