Journal Article

FZJ-2025-02387

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Fundamentals and Applications of Dual‐Frequency Magnetic Particle Spectroscopy: Review for Biomedicine and Materials Characterization

Krause, H.-J.FZJ* ; Engelmann, U. M. (Corresponding author)

2025
Wiley-VCH Weinheim

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Please use a persistent id in citations: doi:10.1002/advs.202416838  doi:10.34734/FZJ-2025-02387

Abstract: Superparamagnetic nanoparticles (MNP) offer exciting applications for engineering and biomedicine in imaging, diagnostics, and therapy upon magnetic excitation. Specifically, if excited at two distinct frequencies f1 and f2, MNP responds with magnetic intermodulation frequencies m·f1 ± n·f2 caused by their nonlinear magnetization. These mixing frequencies are highly specific for MNP properties, uniquely characterizing their presence. In this review, the fundamentals of frequency mixing magnetic detection (FMMD) as a special case of magnetic particle spectroscopy (MPS) are reviewed, elaborating its functional principle that enables a large dynamic range of detection of MNP. Mathematical descriptions derived from Langevin modeling and micromagnetic Monte-Carlo simulations show matching predictions. The latest applications of FMMD in nanomaterials characterization as well as diagnostic and therapeutic biomedicine are highlighted: analysis of the phase of the FMMD signal characterizes the magnetic relaxation of MNP, allowing to determine hydrodynamic size and binding state. Variation of excitation amplitudes or magnetic offset fields enables determining the size distribution of the particles’ magnetic cores. This permits multiplex detection of polydisperse MNP in magnetic immunoassays, realized successfully for various biomolecular targets such as viruses, bacteria, proteins, and toxins. A portable magnetic reader enables portable immunodetection at point-of-care. Future applications toward theranostics are summarized and elaborated.

Note: Open access

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Contributing Institute(s):

1. Bioelektronik (IBI-3)

Research Program(s):

1. 5241 - Molecular Information Processing in Cellular Systems (POF4-524) (POF4-524)

Appears in the scientific report 2025

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