Light induced stimulation and delay of cardiac activity

Hofmann, B.; Eick, S.; Wood, P.; Meffert, S.; Offenhäusser, A.; Maybeck, V.; Ingebrandt, S.; Bamberg, E.

doi:10.1039/c003091k

Journal Article

Light induced stimulation and delay of cardiac activity

Hofmann, B.FZJ* ; Maybeck, V.FZJ* ; Eick, S.FZJ* ; Meffert, S.FZJ* ; Ingebrandt, S. ; Wood, P. ; Bamberg, E. ; Offenhäusser, A.FZJ*

2010
RSC Cambridge

Lab on a chip 10, 2588 - 2596 (2010) [10.1039/c003091k]

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Please use a persistent id in citations: http://hdl.handle.net/2128/15768 doi:10.1039/c003091k

Abstract: This article shows the combination of light activatable ion channels and microelectrode array (MEA) technology for bidirectionally interfacing cells. HL-1 cultures, a mouse derived cardiomyocyte-like cell line, transfected with channelrhodopsin were stimulated with a microscope coupled 473 nm laser and recorded with custom built 64 electrode MEAs. Channelrhodopsin induced depolarization of the cell can evoke action potentials (APs) in single cells. Spreading of the AP over the cell layer can then be measured with good spatiotemporal resolution using MEA recordings. The possibility for light induced pacemaker switching in cultures was shown. Furthermore, the suppression of APs can also be achieved with the laser. Possible applications include cell analysis, e.g. pacemaker interference or induced pacemaker switching, and medical applications such as a combined cardiac pacemaker and defibrillator triggered by light. Since current prosthesis research focuses on bidirectionality, this system may be applied to any electrogenic cell, including neurons or muscles, to advance this field.

Keyword(s): Action Potentials: physiology (MeSH) ; Action Potentials: radiation effects (MeSH) ; Animals (MeSH) ; Cell Line (MeSH) ; Equipment Design (MeSH) ; Equipment Failure Analysis (MeSH) ; Light (MeSH) ; Mice (MeSH) ; Microfluidics: instrumentation (MeSH) ; Myocardial Contraction: physiology (MeSH) ; Myocardial Contraction: radiation effects (MeSH) ; Myocytes, Cardiac: physiology (MeSH) ; Myocytes, Cardiac: radiation effects (MeSH) ; Photic Stimulation: instrumentation (MeSH) ; J

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Note: We gratefully acknowledge the help of Rita Fricke, especially in the cell culture. Marko Banzet and Michael Prompers for processing of the wafers, and all other members of the IBN-2 for fruitful discussions and an excellent working atmosphere. We thank Prof. W. Claycomb for the kind gift of HL-1 cells. Furthermore, Vanessa Maybeck acknowledges financial support by the International Helmholtz Research School on Biophysics and Soft Matter ("BioSoft'').

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Appears in the scientific report 2010

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OpenAccess