Journal Article

PreJuSER-111979

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Controlled In Meso Phase Crystallization -- A Method for the Structural Investigation of Membrane Proteins

Kubicek, J. ; Schlesinger, R. ; Baeken, C.FZJ* ; Büldt, G.FZJ* ; Schäfer, F. ; Labahn, J.FZJ*

2012
PLoS Lawrence, Kan.

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Please use a persistent id in citations: http://hdl.handle.net/2128/7812  doi:10.1371/journal.pone.0035458

Abstract: We investigated in meso crystallization of membrane proteins to develop a fast screening technology which combines features of the well established classical vapor diffusion experiment with the batch meso phase crystallization, but without premixing of protein and monoolein. It inherits the advantages of both methods, namely (i) the stabilization of membrane proteins in the meso phase, (ii) the control of hydration level and additive concentration by vapor diffusion. The new technology (iii) significantly simplifies in meso crystallization experiments and allows the use of standard liquid handling robots suitable for 96 well formats. CIMP crystallization furthermore allows (iv) direct monitoring of phase transformation and crystallization events. Bacteriorhodopsin (BR) crystals of high quality and diffraction up to 1.3 Å resolution have been obtained in this approach. CIMP and the developed consumables and protocols have been successfully applied to obtain crystals of sensory rhodopsin II (SRII) from Halobacterium salinarum for the first time.

Keyword(s): Bacteriorhodopsins: chemistry (MeSH) ; Crystallization (MeSH) ; Crystallography, X-Ray: methods (MeSH) ; Glycerides: chemistry (MeSH) ; Halobacterium salinarum (MeSH) ; Halorhodopsins: chemistry (MeSH) ; Membrane Proteins: chemistry (MeSH) ; Phase Transition (MeSH) ; Protein Stability (MeSH) ; Sensory Rhodopsins: chemistry (MeSH) ; Glycerides ; Halorhodopsins ; Membrane Proteins ; Sensory Rhodopsins ; sensory rhodopsin II protein, archaeal ; monoolein ; Bacteriorhodopsins

Note: Record converted from VDB: 16.11.2012

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Contributing Institute(s):

1. Molekulare Biophysik (ICS-5)
2. Strukturbiochemie (ICS-6)

Research Program(s):

1. BioSoft: Makromolekulare Systeme und biologische Informationsverarbeitung (P45)
2. Funktion und Dysfunktion des Nervensystems (P33)

Appears in the scientific report 2012

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Database coverage:
; ; ; ; BIOSIS Previews ; JCR ; NCBI Molecular Biology Database ; SCOPUS ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection ; Zoological Record

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