Depletion interactions effected by different variants of fd virus

July, C.; Lang, P. R.

doi:10.1021/la104151u

Journal Article

PreJuSER-13066

Depletion interactions effected by different variants of fd virus

July, C.FZJ* ; Lang, P. R.FZJ*

2010
ACS Publ. Washington, DC

Langmuir 26, 18647 - 18651 (2010) [10.1021/la104151u]

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Please use a persistent id in citations: http://hdl.handle.net/2128/4691 doi:10.1021/la104151u

Abstract: The depletion interaction between a probe sphere and a flat wall induced by fd virus is investigated by means of total internal reflection microscopy (TIRM). The viruses serve as a model system for monodisperse, rod-like colloids. We find that the experimental potentials are well described by the first-order density approximation up to an fd content of several overlap concentrations. This is in accordance with higher order density theory as confirmed by numerical calculations. Since the first-order analytical description still holds for all measurements, this exemplifies that higher order terms of the theory are unimportant for our system. Comparing the potentials induced by wild-type fd to those induced by a more rigid fd variant, it can be shown that the influence of the virus stiffness is beyond our experimental resolution and plays only a negligible role for the measured depletion potentials.

Keyword(s): Bacteriophage M13: genetics (MeSH) ; Bacteriophage M13: metabolism (MeSH) ; Colloids (MeSH) ; Microscopy (MeSH) ; Mutation (MeSH) ; Colloids ; J

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Note: We thank Z. Dogic for the gift of the altered Y21M virus stock, P. Lettinga and K. Selinghoff for their help with the virus preparation. The authors acknowledge financial support from the EU through FP7, project Nanodirect (Grant No. NMP4-SL-2008-213948).

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