Journal Article

FZJ-2013-04875

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Keratins as the main component for the mechanical integrity of keratinocytes

Ramms, L. (Corresponding author)FZJ* ; Fabris, G.FZJ* ; Windoffer, R. ; Schwarz, N. ; Springer, R.FZJ* ; Zhou, C. ; Lazar, J. ; Stiefel, S.FZJ* ; Hersch, N.FZJ* ; Schnakenberg, U. ; Magin, T. M. ; Leube, R. E. ; Merkel, R.FZJ* ; Hoffmann, B.FZJ*

2013
Academy Washington, DC

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Please use a persistent id in citations: doi:10.1073/pnas.1313491110

Abstract: For decades, researchers have been trying to unravel one of the key questions in cell biology regarding keratin intermediate filament function in protecting epithelial cells against mechanical stress. For many different reasons, however, this fundamental hypothesis was still unproven. Here we answer this pivotal question by the use of keratin KO cells lacking complete keratin gene clusters to result in total loss of keratin filaments. This lack significantly softens cells, reduces cell viscosity, and elevates plastic cell deformation on force application. Reexpression of single keratin genes facilitates biomechanical complementation of complete cluster loss. Our manuscript therefore makes a very strong case for the crucial contribution of keratins to cell mechanics, with far-reaching implications for epithelial pathophysiology.

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Contributing Institute(s):

1. Biomechanik (ICS-7)

Research Program(s):

1. 453 - Physics of the Cell (POF2-453) (POF2-453)

Appears in the scientific report 2013

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Database coverage:
; BIOSIS Previews ; Current Contents - Agriculture, Biology and Environmental Sciences ; Current Contents - Life Sciences ; JCR ; NCBI Molecular Biology Database ; National-Konsortium ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection ; Zoological Record

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