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| Home > Publications database > Inducing microscopic thermal lesions for the dissection of functional cell networks on a chip |
| Journal Article | FZJ-2015-00648 |
; ; ; ;
2015
RSC
Cambridge
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Please use a persistent id in citations: doi:10.1039/C4LC00805G
Abstract: We present a versatile chip-based method to inflict microscopic lesions on cellular networks or tissue models. Our approach relies on resistive heating of microstructured conductors to impose highly localized thermal stress on specific regions of a cell network. We show that networks can be precisely dissected into individual subnetworks using a microwire crossbar array. To this end, we pattern a network of actively beating cardiomyocyte-like cells into smaller subunits by inflicting thermal damage along selected wires of the array. We then investigate the activity and functional connectivity of the individual subnetworks using a Ca2+ imaging-based signal propagation analysis. Our results demonstrate the efficient separation of func- tional activity between individual subnetworks on a microscopic level. We believe that the presented tech- nique may become a powerful tool for investigating lesion and regeneration models in cellular networks.
; Allianz-Lizenz / DFG ; BIOSIS Previews ; Current Contents - Life Sciences ; Current Contents - Physical, Chemical and Earth Sciences ; IF >= 5 ; JCR ; NCBI Molecular Biology Database ; Nationallizenz
; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection
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