Journal Article

FZJ-2015-01087

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Hierarchical molecular dynamics of bovine serum albumin in concentrated aqueous solution below and above thermal denaturation

Grimaldo, M. (Corresponding Author) ; Roosen-Runge, F. ; Hennig, M. ; Zanini, F. ; Zhang, F. ; Jalarvo, N.FZJ* ; Zamponi, M.FZJ* ; Schreiber, F. ; Seydel, T.

2015
RSC Publ. Cambridge

This record in other databases:      

Please use a persistent id in citations: http://hdl.handle.net/2128/8329  doi:10.1039/C4CP04944F

Abstract: The dynamics of proteins in solution is a complex and hierarchical process, affected by the aqueous environment as well as temperature. We present a comprehensive study on nanosecond time and nanometer length scales below, at, and above the denaturation temperature Td. Our experimental data evidence dynamical processes in protein solutions on three distinct time scales. We suggest a consistent physical picture of hierarchical protein dynamics: (i) self-diffusion of the entire protein molecule is confirmed to agree with colloid theory for all temperatures where the protein is in its native conformational state. At higher temperatures T > Td, the self-diffusion is strongly obstructed by cross-linking or entanglement. (ii) The amplitude of backbone fluctuations grows with increasing T, and a transition in its dynamics is observed above Td. (iii) The number of mobile side-chains increases sharply at Td while their average dynamics exhibits only little variations. The combination of quasi-elastic neutron scattering and the presented analytical framework provides a detailed microscopic picture of the protein molecular dynamics in solution, thereby reflecting the changes of macroscopic properties such as cluster formation and gelation.

Keyword(s): Health and Life (1st) ; Health and Life (1st) ; Soft Condensed Matter (2nd)

---

Contributing Institute(s):

1. Neutronenstreuung (ICS-1)
2. Neutronenstreuung (Neutronenstreuung ; JCNS-1)
3. JCNS-SNS (Jülich Centre for Neutron Science JCNS (JCNS) ; JCNS)
4. JCNS-FRM-II (JCNS (München) ; Jülich Centre for Neutron Science JCNS (München) ; JCNS-FRM-II)

Research Program(s):

1. 551 - Functional Macromolecules and Complexes (POF3-551) (POF3-551)
2. 6G4 - Jülich Centre for Neutron Research (JCNS) (POF3-623) (POF3-623)
3. 6215 - Soft Matter, Health and Life Sciences (POF3-621) (POF3-621)

Experiment(s):

1. Measurement at external facility

Appears in the scientific report 2015

---

Database coverage:
; ; ; Allianz-Lizenz / DFG ; Current Contents - Physical, Chemical and Earth Sciences ; IF < 5 ; JCR ; NCBI Molecular Biology Database ; Nationallizenz ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection

---