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| Home > Publications database > Comparative analysis of gene expression data after exposure to I-123-iododeoxyuridine, γ-rays and α-particles |
| Poster (After Call) | FZJ-2015-02036 |
; ; ;
2014
Please use a persistent id in citations: http://hdl.handle.net/2128/16213
Abstract: Gene expression analysis was carried out in Jurkat cells in order to identify robust candidate genes showing significant gene expression alterations allowing the discrimination of radiation qualities.Equi-effect radiation doses, i.e. radiation doses and exposure conditions causing the same biological effect level, were determined with regard to micronucleus formation, γ-H2AX foci signal intensity and apoptosis induction after γ-irradiation, α-irradiation and exposure to the Auger electron emitter I-123-iododeoxyuridine (123IdU). Microarray gene expression analysis was performed after exposure to equi-effect radiation doses of all three radiation qualities. Candidate genes for the discrimination of radiation quality have to show a significant expression change after exposure to a specific radiation quality and display no altered gene regulation or a conversely regulation in response to exposure to the other radiation qualities investigated. Gene expression of selected candidate genes was validated via qRT-PCR. Biological processes and pathways of significantly regulated genes were subsequently analyzed.At equi-effect doses the results of the gene expression analysis showed that 359, 598 and 1339 genes are significantly regulated after exposure to 123IdU, α-particles and γ-rays, respectively. Only 4, 1 and 1 gene(s) are identified allowing a reliable discrimination between γ- vs. 123IdU-exposure, γ- vs. α-radiation and α- vs. 123IdU-exposure, respectively.γ-rays induce pronounced alterations in gene expression in Jurkat cells when compared to 123IdU and α-particles at equi-effect radiation doses. Gene expression analysis in Jurkat cells might suggest that the discrimination of different radiation qualities by means of gene expression is possible.
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